25OH Vitamin D Total ELISA

PROMOCJA - PROSIMY O KONTAKT

Darmowa wysyłka

 The  25-Hydroxyvitamin D Total ELISA is powered by unique  monoclonal antibodies that express  100 % reactivity against 25 OH Vitamin D3  and 83% reactivity against 25OH Vitamin D2.
All In One technology. No extraction step, displacement solution directly into the well. Results in less than 4 hours. Calibrated against ID-LC/MS-MS (reference method).  Bar codes on all components.

The Gentaur/GDMS 25-OH Vitamin D Total ELISA is a solid phase Enzyme Linked Immunosorbent Assay performed on microtiterplates. During a first 2 hours incubation step, at room temperature, total 25-OH Vitamin D (D2 and D3) present in calibrators, controls and samples is dissociated from binding serum proteins to fix on binding sites of a specific monoclonal antibody. After 1 washing step, a fixed amount of 25-OH Vitamin D-labelled with biotin in presence of horseradish peroxidise (HRP), compete with unlabelled 25-OH Vitamin D2 and 25-OH Vitamin D3 present on the binding sites of the specific monoclonal antibody. After a 30 minutes incubation at room temperature, the microtiterplate is washed to stop the competition reaction. The Chromogenic solution (TMB) is added and incubated for 15 minutes. The reaction is stopped with the addition of Stop Solution and the microtiterplate is then read at the appropriate wavelength. The amount of substrate turnover is determined colourimetrically by measuring the absorbance, which is inversely proportional to the total 25-OH Vitamin D (D2 and D3) concentration. A calibration curve is plotted and the total 25-OH Vitamin D (D2 and D3) concentrations of the samples are determined by dose interpolation from the calibration curve.

Porównanie protokołów: