QuantiChrom™ LDH Cytotoxicity Assay Kit

Application
• For quantitative determination of cytotoxicity based on lactate dehydrogenase released into cell culture medium. Evaluation of toxic compounds, toxins, detergents and environmental pollutants.

Key Features
• Safe. Non-radioactive assay (cf. chromium release assay).

• Fast. High-throughput assay using 96-well plates allows simultaneous processing tens of thousands of samples per day.

• Homogeneous and convenient. "Mix-incubate-measure" type assay. No wash steps are involved.

• Robust and amenable to HTS. Can be readily automated with HTS liquid handling systems.

Method
• OD565nm

Samples
• Cell culture

Species
• All

Procedure
• 30 min

Size
• 100 tests

Shelf Life
• 6 months

More Details
• Lactate dehydrogenase(LDH) is an oxidoreductase which catalyzes the interconversion of lactate and pyruvate. Cytotoxic compounds often compromise cell membrane integrity by inducing apoptosis or necrosis. LDH is a stable cytosolic enzyme that upon membrane damage is released into the cellular environment. Therefore, LDH is often measured to evaluate the presence of tissue or cell damage. The colorimetric LDH release assay is a simple and robust method to assess cytotoxic effects on cells by measuring the activity of LDH in cell culture supernatant. The assay is based on the reduction of the tetrazolium salt MTT to a formazan dye which exhibits an absorption maximum at 565 nm.

Shipping: RT
Storage: 4, -20°C